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About This Item
Full Description
1.1 This practice is intended to be used to reduce the cytotoxic level of the virus-test product mixture prior to assaying for viral infectivity. It is used in conjunction with evaluations of the virucidal efficacy of disinfectant solutions, wipes, trigger sprays, or pressurized disinfectant spray products intended for use on inanimate, nonporous environmental surfaces. This practice may also be used in the evaluation of hygienic handwashes/handrubs, or for other special applications. The practice may be employed with all viruses and host systems.
1.3 This practice utilizes gel filtration technology. The effectiveness of the practice is dependent on the ratio of gel bed volume to sample size and uniformity in the preparation of columns as well as the conditions of entrifugation. The effectiveness of this practice is maximized by investigator practice and experience with gel filtration techniques.
1.4 This practice will aid in the reduction, but not necessarily elimination, of test product toxicity while preserving the titer of the input virus.
Document History
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ASTM E1482-23
Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization- Most Recent
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ASTM E1482-12(2017)
Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization- Historical Version
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ASTM E1482-12
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Standard Practice for Use of Gel Filtration Columns for Cytotoxicity Reduction and Neutralization- Historical Version
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ASTM E1482-04
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations- Historical Version
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ASTM E1482-92(2004)
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations- Historical Version
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ASTM E1482-92(1998)
Standard Test Method for Neutralization of Virucidal Agents in Virucidal Efficacy Evaluations- Historical Version