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About This Item
Full Description
1.1 This guide covers procedures for obtaining laboratory data concerning the acute effects of a test material on embryos and the resulting larvae of echinoid embryos (sea urchins and sand dollars) during static 48- to 96-h exposures. These procedures have generally been used with U.S. East Coast (Arbacia punctulata and Strongylocentrotus droebachiensis ) (1)3 and West Coast species (Strongylocentrotus purpuratus, S. droebachiensis, and Dendraster excentricus) (2). The basic procedures described in this guide first originated in Japan and Scandanavia (3), and parallel procedures have been used with foreign species, especially in Japan and the Mediterranean (4). These procedures will probably be useful for conducting static acute toxicity tests with embryos of other echinoid species, although modifications might be necessary.
1.3 These procedures are applicable to most chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct acute tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, surface waters and sediments (Annex A1). Renewal tests might be preferable to static tests for materials that have a high oxygen demand, are highly volatile, are rapidly transformed biologically or chemically in aqueous solution, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.
1.5 This guide is arranged as follows:
| Section |
Scope | |
Referenced Documents | |
Terminology | |
Summary of Guide | |
Significance and Use | |
Apparatus | |
Facilities | |
Construction Materials | |
Test Chambers | |
Cleaning | |
Acceptability | |
Safety Precautions | |
Dilution Water | |
Requirements | |
Source | |
Treatment | |
Characterization | |
Test Material | |
General | |
Stock Solution | |
Test Concentration(s) | |
Test Organisms | |
Species | |
Age | |
Source of Embryos | |
Handling | |
Test Animal Source and Condition | |
Spawning and Fertilization | |
Quality | |
Procedure | |
Experimental Design | |
Dissolved Oxygen | |
Temperature | |
Beginning the Test | |
Feeding | |
Duration of Test | |
Biological Data | |
Control Performance | |
Other Measurements | |
Analytical Methods | |
Acceptability of Test | |
Calculation of Results | |
Report | |
Keywords | |
Annex |
|
Sediment Tests |
1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations. Specific precautionary statements are given in Section 7.
Document History
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ASTM E1563-21a
Standard Guide for Conducting Short-Term Chronic Toxicity Tests with Echinoid Embryos- Most Recent
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ASTM E1563-21
Standard Guide for Conducting Short-Term Chronic Toxicity Tests with Echinoid Embryos- Historical Version
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ASTM E1563-98(2012)
viewing
Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos- Historical Version
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ASTM E1563-98(2004)e1
Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos- Historical Version
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ASTM E1563-98(2004)
Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos- Historical Version
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ASTM E1563-98
Standard Guide for Conducting Static Acute Toxicity Tests with Echinoid Embryos- Historical Version