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The objective of this study was to elucidate some of the mechanisms responsible for the passage of viruses, organic solutes, and inorganic solutes through nanofiltration (NF) membranes by characterizing the pore size distribution (PSD) of the membranes. Microbial and chemical materials are discussed along with the lab-scale membrane system; experimental conditions, analytical methods, atomic force microscopy (AFM), transmission electron microscopy (TEM), and, ultramicrotomy. Includes 4 references, figures.