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About This Item
Full Description
This document specifies a method for the determination of individual intact glucosinolates in feed materials including oilseeds and oilseed products and in compound feeds by high performance liquid chromatography (HPLC) coupled with tandem mass spectrometry (MS/MS). The method specified in this document has been successfully validated by collaborative trial in the following matrices: rape seed, camelina seed, Brassica oleracea seeds, mixed oilseeds, rape seed flakes, compound feed for bovine, porcine and poultry. The method is applicable for the quantitative determination of epiprogroitrin, glucoalyssin, glucoarabin, glucobrassicanapin, glucobrassicin, glucocamelinin, glucoerucin, glucoiberin, gluconapin, gluconapoleiferin, gluconasturtiin, glucoraphanin, glucoraphenin, glucotropaeolin, homoglucocamelinin, 4-hydroxyglucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin, progoitrin, sinalbin and sinigrin. The concentration ranges tested in the collaborative trial for each individual glucosinolate and for the total glucosinolate content are summarized in Table 1. [table not represented]
Document History
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DANSK DSF/PREN 17853
Animal feeding stuffs: Methods of sampling and analysis – Determination of intact glucosinolates in rapeseed by LC-MS/MS- Historical Version
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DANSK DSF/FPREN 17853
Animal feeding stuff: Methods of sampling and analysis – Determination of intact glucosinolates in feed materials and compound feed by LC-MS/MS- Historical Version
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DANSK DS/EN 17853
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Animal feeding stuff: Methods of sampling and analysis – Determination of intact glucosinolates in feed materials and compound feed by LC-MS/MS- Most Recent