AWWA WQTC71353
Real-time PCR Compared with Culture Method for the Analysis of Legionella in Cooling Towers: A Routine Lab View
Conference Proceeding by American Water Works Association , 11/01/2009
Cantin, Philippe; Guillement, Thibault
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About This Item
Full Description
The
objectives of this study were to validate a qPCR-based test method and a
commercial test kit for the detection and quantification of Legionella spp. and Legionella pneumophila in
water using the AFNOR XP T90-471 method, and to compare qPCR with culture method
NF T90-431 for Legionella testing in 38 cooling tower water samples and 5 hot water
system samples. In this study, qPCR in conjunction with the commercial test kits used in
the present work were relatively simple to use. However, the detection and quantification
limits were affected by PCR inhibitors in the DNA extracts from cooling water samples.
Therefore, it appears that qPCR cannot become the sole method for Legionella testing offered
by the laboratory, and the culture method remains essential. Includes 9 references, tables, figures.
