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ISO/TS 19006:2016

Nanotechnologies - 5-(and 6)-Chloromethyl-2,7 Dichloro-dihydrofluorescein diacetate (CM-H2DCF-DA) assay for evaluating nanoparticle-induced intracellular reactive oxygen species (ROS) production in RAW 264.7 macrophage cell line

standard by International Organization for Standardization (Technical Standard) , 08/01/2016

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Full Description

ISO/TS 19006:2016 describes how to test and evaluate results obtained from in vitro ROS generation in RAW 264.7 macrophage cells exposed to nano-objects, nanoparticles, their aggregates and agglomerates using the CM-H2DCFDA assay.

The protocol in ISO/TS 19006:2016 is limited to use of a 24 well plate so if other plates were to be used, volumes would need to be adjusted and the protocol steps validated to ensure confidence in the test results.

 

Product Details

Published:
08/01/2016
Number of Pages:
28
File Size:
1 file , 1.5 MB
Same As:
BS PD ISO/TS 19006:2016
Note:
This product is unavailable in Russia, Ukraine, Belarus