1.1 This test method covers screening procedures for the determining the biodegradation potential of organic chemicals under methanogenic conditions using an inoculum from anaerobic sewage treatment. The conditions described in this test method are not necessarily optimal for biodegradation, since it involves a dilute inoculum and a relatively high concentration of test chemical.

1.2 The test method is applicable to most organic chemicals, assuming that they can be accurately dosed into the test systems. The test is not applicable to chemicals that are inhibitory at the concentration used in the test or those whose volatility generates significant head pressure in the test vessels.

1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 8.