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This collection of viewgraphs presents information on the viability of Cryptosporidium parvum oocysts exposed to carbon dioxide and freeze-thaw cycles. Topics covered include: an overview of cryptosporidium parvum oocysts; susceptibility of groundwater contamination; CO2 injection process; effects of freeze-thaw mechanism and of CO2 at room temperature; experiment design; cryptonator device; sentinel design; laboratory research; detecting oocyst state; oocyst viability categories; room temperature tests; and, freeze-thaw tests.