This document specifies general requirements for DNA sequencing method performance in the detection and identification of animal species in food and feed products. Performance requirements are limited to Sanger and next generation sequencing (NGS), including second and third generation sequencing, for analysis of single species products and multispecies products. This document is applicable to DNA sequences for mammals, birds, fish, molluscs, crustaceans, amphibians, reptiles and insects, and to the validation of the applicable methods. Methods for DNA species quantification are not considered under the scope of this document.